毛建草愈伤组织诱导及植株再生

田旭平, 岳康杰, 王佳丽, 刘慧欣, 史子尹, 亢红伟

Callus Induction and Plant Regeneration of Dracocephalum rupestre

Xuping Tian, Kangjie Yue, Jiali Wang, Huixin Liu, Ziyin Shi, Hongwei Kang

表1 细胞分裂素对叶片愈伤组织诱导的影响

Table 1 Effects of cytokinins on callus induction from leaves

Type 6-BA (mg∙L-1) TDZ (mg∙L-1) KT (mg∙L-1) Occurrence time (d) Induction rate (%) Callus growth state Leaves of field planted individual 0.5 - - 10.25±4.65 a 40.36±3.17 b Yellow with a firm and dense texture 1.0 - - 7.58±1.29 b 65.21±9.94 a Pale yellow-green, loose, and a softer texture 2.0 - - 6.92±1.17 b 67.07±9.05 a Pale yellow, loose and watery in appearance, with a soft texture - 0.5 - 16.25±3.39 a 37.98±5.73 b Green, loose, and soft in texture, with a loose consistency - 1.0 - 15.83±3.58 a 40.17±7.53 b Green, loose, with white spots, a soft texture - 2.0 - 11.50±2.88 b 62.53±6.66 a Green, loose, with white spots, a soft texture - - 0.5 9.50±2.60 a 35.19±6.73 b Yellow, compact, with a somewhat hard texture - - 1.0 8.17±2.44 ab 57.06±9.06 a Brownish-yellow, compact, with a soft texture - - 2.0 6.67±1.45 b 42.53±5.84 b Brown, loose, resembling water stains, with a soft texture Leaves of tissue-cultured seedling 0.5 - - 11.08±2.69 a 47.33±8.53 b Yellow with a firm and dense texture 1.0 - - 10.33±1.48 a 55.76±7.85 ab Yellow-green, loose, with a relatively soft texture 2.0 - - 8.17±1.27 b 68.01±6.46 a Yellow-green, loose, with a relatively soft texture - 0.5 - 14.17±2.99 a 33.63±5.79 b Green, loose, with a soft texture - 1.0 - 12.33±1.03 ab 45.36±9.64 ab Green, compact, with a hard texture - 2.0 - 10.33±2.63 b 55.61±9.07 a Green, loose, with a relatively soft texture - - 0.5 12.92±3.62 a 40.75±8.32 b Pale yellow, loose, with a hard texture - - 1.0 11.25±2.65 a 63.59±7.07 a Pale yellow, loose, with a softer texture - - 2.0 8.83±1.58 b 52.29±9.41 ab Pale yellow, loose, and loosely resembling water stains in texture