伊藤杂种‘和谐’组培快繁体系的建立

康敏, 张美莹, 齐秀双, 佟宁宁, 李旸, 舒庆艳, 刘政安, 吕长平, 彭丽平

Establishment of a Fast Breeding System for Itoh Hybrid ‘He Xie’ in Tissue Culture

Min Kang, Meiying Zhang, Xiushuang Qi, Ningning Tong, Yang Li, Qingyan Shu, Zheng’an Liu, Changping Lü, Liping Peng

图2 ‘和谐’试管苗生根和驯化移栽的生长表现 (A) 用于生根诱导的单芽(bar=2 cm); (B) 春化30天(冷处理8天, 根诱导30天, 根伸长20天后于4°C冷藏30天)的生根苗(bar= 2 cm); (C)-(F) 不同生根质量的试管苗(C: 三级苗; D: 二级苗; E, F: 一级苗) (bars=2 cm); (G)-(I) 驯化60天不同生根质量的移栽苗(G: 三级苗; H: 二级苗; I: 一级苗) (bars=3 cm)

Figure 2 The growth performance for rooting and domesticated transplanting of ‘He Xie’ tube plantlet (A) Single shoots used for root induction (bar=2 cm); (B) Rooted seedlings after 30 d of vernalization (cold treatment for 8 days, root induction for 30 days, and root elongation for 20 days, cold storage at 4°C for 30 days) (bar=2 cm); (C)-(F) The test tube plantlets with different rooting qualities (C: Tertiary; D: Secondary; E, F: Primary) (bars=2 cm); (G)-(I) The transplanted seedlings of different rooting quality for domesticating 60 d (G: Tertiary; H: Secondary; I: Primary) (bars=3 cm)