蒙古冰草肉桂醇脱氢酶基因序列鉴定及功能分析

王贺萍, 孙震, 刘雨辰, 苏彦龙, 杜锦瑜, 赵彦, 赵竑博, 王召明, 苑峰, 刘亚玲, 吴振映, 何峰, 付春祥

Sequence Identification and Functional Analysis of Cinnamyl Alcohol Dehydrogenase Gene from Agropyron mongolicum

Heping Wang, Zhen Sun, Yuchen Liu, Yanlong Su, Jinyu Du, Yan Zhao, Hongbo Zhao, Zhaoming Wang, Feng Yuan, Yaling Liu, Zhenying Wu, Feng He, Chunxiang Fu

图4 AmCAD重组蛋白在大肠杆菌中的异源表达(A)及不同温度(B)和pH (C)下的酶学性质 1: 蛋白Marker; 2: 上清液; 3: 沉淀; 4: 流出液; 5: 纯化蛋白

Figure 4 Heterologous expression (A) and enzymatic properties of AmCAD recombinant protein in Escherichia coli at different temperatures (B) and pH (C) 1: Marker; 2: Supernatant; 3: Precipitant; 4: Effluent; 5: Purified protein