蒙古冰草肉桂醇脱氢酶基因序列鉴定及功能分析

王贺萍, 孙震, 刘雨辰, 苏彦龙, 杜锦瑜, 赵彦, 赵竑博, 王召明, 苑峰, 刘亚玲, 吴振映, 何峰, 付春祥

Sequence Identification and Functional Analysis of Cinnamyl Alcohol Dehydrogenase Gene from Agropyron mongolicum

Heping Wang, Zhen Sun, Yuchen Liu, Yanlong Su, Jinyu Du, Yan Zhao, Hongbo Zhao, Zhaoming Wang, Feng Yuan, Yaling Liu, Zhenying Wu, Feng He, Chunxiang Fu

图3 蒙古冰草AmCAD基因扩增和蛋白结构分析 (A) AmCAD电泳图(M: DNA ladder; 1: 扩增序列); (B) 蒙古冰草与其它物种CAD氨基酸序列比对(构成底物结合口袋的残基用三角形(▲)标记); (C) AmCAD蛋白质三维结构模型和底物结合口袋(底部亚基的核苷酸结合和催化结构域分别呈黄色和蓝色, 在上部亚基中的对应结构分别以绿色和深蓝色显示, 锌离子用灰色球体表示)

Figure 3 Amplification of Agropyron mongolicum AmCAD and protein structure analysis (A) AmCAD gel map (M: DNA ladder; 1: Amplification sequence); (B) Comparison of CAD amino acid sequences among A. mongolicum and other species (the residue forming the substrate binding pocket is marked with a triangle (▲)); (C) Three-dimensional protein structure model and substrate binding pocket of AmCAD (the nucleotide binding and catalytic domains of the bottom subunit are colored yellow and blue, respectively, the corresponding compounds in the upper subunit are colored green and dark blue, while the zinc ions are indicated by gray spheres)