蒙古冰草肉桂醇脱氢酶基因序列鉴定及功能分析

王贺萍, 孙震, 刘雨辰, 苏彦龙, 杜锦瑜, 赵彦, 赵竑博, 王召明, 苑峰, 刘亚玲, 吴振映, 何峰, 付春祥

Sequence Identification and Functional Analysis of Cinnamyl Alcohol Dehydrogenase Gene from Agropyron mongolicum

Heping Wang, Zhen Sun, Yuchen Liu, Yanlong Su, Jinyu Du, Yan Zhao, Hongbo Zhao, Zhaoming Wang, Feng Yuan, Yaling Liu, Zhenying Wu, Feng He, Chunxiang Fu

表2 AmCAD重组蛋白催化不同底物的酶动力学特性

Table 2 Enzyme kinetic characteristics of different substrates catalyzed by recombinant AmCAD

Substract	K_m (μmol·L^-1)	V_max(μmol·L^-1·s^-1)	K_cat (·s⁻¹)	K_cat/K_m(μmol·L^-1·s^-1)
Coniferylaldehyde	8.94±0.15	6.87±0.008	441.37±0.53	49.35±0.90
Sinapaldehyde	10.35±2.14	7.18±0.43	461.51±27.94	45.26±6.69
p-coumaraldehyde	35.35±3.74	15.21±1.60	977.60±102.97	27.65±0.01