基于正交实验的赤苍藤组培快繁体系建立

张尚文, 黄诗宇, 杨天为, 李婷, 张向军, 高曼熔

Establishment of a Tissue Culture and Rapid Propagation System for Erythropalum scandens Based on Orthogonal Test

Shangwen Zhang, Shiyu Huang, Tianwei Yang, Ting Li, Xiangjun Zhang, Manrong Gao

图1 赤苍藤外植体离体再生体系的建立 (A) 诱导初代苗; (B) 叶(1)和茎段(2)愈伤组织的诱导; (C) 增殖培养愈伤组织; (D)-(F) 愈伤组织分化; (G), (H) 切单壮苗培养; (I) 生根培养; (J) 生根苗移栽; (K) 移栽成活180天(bar=10 cm); (L) 移栽成活360天(bar=35 cm)。(A)-(J) Bars=2 cm

Figure 1 Establishment of plant regeneration of cotyledons of Erythropalum scandens (A) Induction primary seedlings; (B) Callus induction of leaf (1) and stem (2); (C) Proliferation culture callus; (D)-(F) Callus differentiation; (G), (H) Cut single seedling culture; (I) Rooting culture; (J) Seedlings were transplanted; (K) Transplant and survive for 180 days (bar=10 cm); (L) Transplant and survive for 360 days (bar=35 cm). (A)-(J) Bars=2 cm