利用转基因毛状根高效培育大豆嵌合植株的方法

陈佳欣, 梅浩, 黄彩翔, 梁宗原, 全依桐, 李东鹏, 布威麦尔耶姆·赛麦提, 李欣欣, 廖红

A Highly Efficient Method to Generate Chimeric Soybean Plant with Transgenic Hairy Roots

Jiaxin Chen, Hao Mei, Caixiang Huang, Zongyuan Liang, Yitong Quan, Dongpeng Li, Buweimaieryemu·Saimaiti , Xinxin Li, Hong Liao

图1 发根农杆菌诱导大豆下胚轴转基因毛状根产生嵌合植株流程 (A) 萌发4天后的大豆幼苗(bar=2 cm); (B) 在下胚轴处以45°角切开形成伤口(bar=1 cm); (C) 从培养皿中收集发根农杆菌(bar=1 cm); (D) 在伤口处涂菌(bar=1 cm); (E) 共培养(bar=1 cm); (F) 将外植体移入蛭石中(bar=5 cm); (G) 培养10天后诱导出愈伤组织(bar=1 cm); (H) 诱导14天后毛状根形成(bar=1 cm); (I) 毛状根生长20天后的状态(bar=2 cm); (J) I图的局部放大(bar=2 cm)

Figure 1 Procedure of generating chimeric soybean plants with Agrobacterium rhizogenes induced hypocotyl transgenic hairy roots (A) Soybean seedlings at 4 d post germination (bar=2 cm); (B) Hypocotyl was cut to form wound site with an angle of 45° (bar=1 cm); (C) A. rhizogenes were collected in petri dishes (bar=1 cm); (D) Apply bacteria to the wound (bar=1 cm); (E) Co-cultivation (bar=1 cm); (F) Inoculated explants were planted into vermiculite (bar=5 cm); (G) Callus came out after 10 d of cultivation (bar=1 cm); (H) Hairy roots emerged at 14 d post induction (bar=1 cm); (I) Growth performance of hairy roots after 20 d of growth (bar=2 cm); (J) Partial enlarged view of image I (bar=2 cm)