点击化学反应在植物细胞标记中的应用

张御格, 袁笑妍, 张贵芳, 李雨健, 殷金环, 林金星, 李晓娟

The Application of Click Chemistry Reactions in Plant Cell Labeling

Yuge Zhang, Xiaoyan Yuan, Guifang Zhang, Yujian Li, Jinhuan Yin, Jinxing Lin, Xiaojuan Li

图2 点击化学标记在细胞壁聚糖中应用流程图 图中非天然糖指细胞壁中的单糖组分, 不特指具体糖类。① 外源单糖类似物通过聚糖合成的补救途径代谢进入细胞; ② 含荧光团的探针与沉积在细胞壁上并含有单糖类似物的聚糖发生点击反应; ③ 荧光成像检测单糖类似物在植物体内的排布, 如下方荧光成像图所示植物细胞壁中的果胶RG-II; ④ 荧光显微镜成像点击化学标记的果胶在植物根中的排布(bar=50 μm)。

Figure 2 The flow diagram of applying click chemistry to labeling cell wall glycan The non-natural sugars in the figure refer to the monosaccharide components in the cell wall, and do not refer to specific sugar molecules. ① Exogenous monosaccharide analogues are metabolized into cells through the remedial glycan synthesis pathway; ② Probes containing fluorophores click with glycans that contain monosaccharide analogues and are deposited on the cell wall; ③ Fluorescence is detected to investigate the arrangement of monosaccharide analogues in plants, as shown in the fluorescent image of pectin RG-II in plant cell wall; ④ Fluorescence microscopy imaging of pectin distribution in plant roots labelled by click chemistry (bar=50 μm).