适配体技术及其在植物科学研究中的应用

李奕, 张曦, 袁艳辉, 公丕昌, 林金星

Aptamers and Their Applications in Plant Science Researches

Yi Li, Xi Zhang, Yanhui Yuan, Pichang Gong, Jinxing Lin

图2 通过人工合成多肽适配体库筛选靶蛋白最适多肽适配体流程 利用人工合成肽库的方式, 体外筛选得到与靶蛋白结合的最适多肽适配体。在体外向植物表面喷施多肽适配体, 多肽适配体进入植物细胞, 影响靶蛋白与其它蛋白的相互作用, 从而抑制靶蛋白的功能。

Figure 2 The process of selecting the most suitable peptide aptamer for the target protein through the synthetic peptide aptamer library The optimal peptide aptamer binding to the target protein was screened in vitro by using a synthetic peptide library. Peptide aptamers are sprayed on the surface of plants in vitro, and peptide aptamers enter plant cells to affect the interaction between target proteins and other proteins, thereby inhibiting the function of target proteins.