适配体技术及其在植物科学研究中的应用

李奕, 张曦, 袁艳辉, 公丕昌, 林金星

Aptamers and Their Applications in Plant Science Researches

Yi Li, Xi Zhang, Yanhui Yuan, Pichang Gong, Jinxing Lin

图1 适配体的分类、筛选及其作用靶标 适配体分为核酸适配体和多肽适配体两类。核酸适配体包括DNA适配体和RNA适配体, 筛选方式主要包括配体指数富集系统进化(SELEX)技术以及基于SELEX的微流控芯片技术、镜像筛选技术和计算机建模筛选等。多肽适配体的筛选方式主要包括噬菌体展示技术、化学合成随机多肽库筛选和分子对接模拟筛选等。适配体的靶标主要包括细胞、细菌、病毒、DNA、RNA和蛋白质等。

Figure 1 The classification, screening and targets of aptamers The aptamers can be divided into nucleic acid aptamers and peptide aptamers. The nucleic acid aptamers include DNA aptamers and RNA aptamers, and the screening methods mainly include systematic evolution of ligands by exponential enrichment (SELEX) technology, and SELEX based microfluidic chip, mirror-image and computer modeling. Peptide aptamers can be screened by phage display, random peptide library and molecular docking. The targets of aptamers are mainly involved in cells, bacteria, virus, DNA, RNA, and proteins.