硝酸盐转运蛋白NRT2在植物中的功能及分子机制研究进展

黄慧梅, 高永康, 台玉莹, 刘超, 曲德杰, 汤锐恒, 王幼宁

Research Advances in Elucidating the Function and Molecular Mechanism of the Nitrate Transporter 2 (NRT2) Proteins in Plants

Huang Huimei, Gao Yongkang, Tai Yuying, Liu Chao, Qu Dejie, Tang Ruiheng, Wang Youning

图2 拟南芥硝酸盐转运蛋白AtNRT2.1的分子调控机制总结 双螺旋表示AtNRT2.1的启动子区域, 受到多个转录因子的直接调控。其中蓝色矩形代表转录抑制子, 绿色椭圆形代表转录激活子。定位在质膜上的AtNRT2.1蛋白通过与NURK1激酶或CEPH磷酸酶的互作发生磷酸化或去磷酸化修饰。目前明确调控硝酸根吸收活性的磷酸化位点包括N端Ser11、Ser21、Ser28及C端Ser501。

Figure 2 A summary of the molecular regulatory mechanism of nitrate transporter AtNRT2.1 in Arabidopsis The double stand represents the promoter region of AtNRT2.1. Several transcription factors are involved in repressing or activating the expression of AtNRT2.1. The blue rectangle and green ellipse indicate transcriptional repressor and activator, respectively. AtNRT2.1 is located in the plasma membrane and could interact with NURK1 kinase or CEPH phosphatase. Phosphorylation at N-terminal Ser11, Ser21, Ser28, and C-terminal Ser501 are critical for nitrate uptake activity of AtNRT2.1.