根癌农杆菌介导万寿菊遗传转化体系的建立

余晓敏, 王亚琴, 刘雨菡, 易庆平, 程文翰, 朱钰, 段枫, 张莉雪, 何燕红

Establishment of Agrobacterium tumefaciens-mediated Genetic Transformation System of Marigold (Tagetes erecta)

Yu Xiaomin, Wang Yaqin, Liu Yuhan, Yi Qingping, Cheng Wenhan, Zhu Yu, Duan Feng, Zhang Lixue, He Yanhong

图3 万寿菊遗传转化流程图 (A) 万寿菊复叶; (B) 小叶培养; (C) 小叶诱导形成愈伤组织; (D) 瞬时转化叶片GUS染色; (E) 抗性芽; (F) 抗性苗; (G) 抗性苗生根培养; (H) 阳性苗; (I) 阳性苗移栽。(A)-(D), (G)-(I) Bars=1 cm; (E), (F) Bars=0.5 cm

Figure 3 Genetic transformation process diagram of marigold (A) Marigold compound leaves; (B) Leaflet culture; (C) Leaflet-induced calli; (D) GUS staining of transiently transformed leaves; (E) Resistance buds; (F) Resistant seedlings; (G) Rooting culture of resistant seedlings; (H) Positive seedlings; (I) Transplantation of positive seedlings. (A)-(D), (G)-(I) Bars=1 cm; (E), (F) Bars=0.5 cm