根癌农杆菌介导万寿菊遗传转化体系的建立

余晓敏, 王亚琴, 刘雨菡, 易庆平, 程文翰, 朱钰, 段枫, 张莉雪, 何燕红

Establishment of Agrobacterium tumefaciens-mediated Genetic Transformation System of Marigold (Tagetes erecta)

Yu Xiaomin, Wang Yaqin, Liu Yuhan, Yi Qingping, Cheng Wenhan, Zhu Yu, Duan Feng, Zhang Lixue, He Yanhong

图1 菌株类型对万寿菊遗传转化效率的影响 (A) 3种菌株的GUS基因瞬时表达和抗性愈伤组织示意图(bars=1 cm); (B) 菌株类型对GUS基因瞬时表达效率及抗性愈伤组织诱导效率的影响(相同颜色柱形图上不同小写字母表示在0.05水平差异显著)

Figure 1 Effects of strain types on genetic transformation efficiency of marigold (A) Schematic diagram of transient expression of GUS gene and resistant callus in three strains (bars=1 cm); (B) Effect of strain types on transient expression efficiency of GUS gene and induction rate of resistant calli (different lowercase letters on the same color histogram indicate significant differences at 0.05 level)