沙棘hrh-miRn458靶向转录因子WRI1调控油脂合成

于淼, 阮成江, 丁健, 李景滨, 卢顺光, 温秀凤

Hrh-miRn458 Regulates Oil Biosynthesis of Sea Buckthorn via Targeting Transcription Factor WRI1

Yu Miao, Ruan Chengjiang, Ding Jian, Li Jingbin, Lu Shunguang, Wen Xiufeng

图4 pmirGLO-WRI1-WT/MUT+hrh-miRn458序列鉴定及荧光素酶活性分析 (A) pmirGLO-WRI1-WT/MUT+hrh-miRn458 PCR产物序列, 黄色部分为突变序列; (B) pmirGLO-WRI1-WT/MUT质粒酶切鉴定(a: XhoI酶切质粒; b: 野生型DNA质粒; c: NheI酶切质粒; d: 突变型DNA质粒); (C) 荧光素酶活性分析(1: pmirGLO- WRI1-WT+mimics NC质粒; 2: pmirGLO-WRI1-WT+hrh-miRn458 mimics质粒; 3: pmirGLO-WRI1-MUT+mimics NC质粒; 4: pmirGLO-WRI1-MUT+hrh-miRn458 mimics质粒)。*** 差异极显著(P<0.001); ns: 无显著差异(P>0.05)。

Figure 4 Sequences identification of pmirGLO-WRI1-WT/MUT+hrh-miRn458 and activity analysis of dual luciferase (A) Sequences of PCR products of pmirGLO-WRI1-WT/MUT+hrh-miRn458 from plasmid, yellow letters indicated mutation sequence; (B) Identification of pmirGLO-WRI1-WT/MUT plasmid digestion (a: Plasmid digested by XhoI enzyme; b: Plasmid DNA of WT; c: Plasmid digested by NheI enzyme; d: Plasmid DNA of MUT); (C) Activity analysis of dual luciferase (the numbers of 1, 2, 3 and 4 represents plasmid pmirGLO-WRI1-WT+mimics NC, pmirGLO-WRI1-WT+hrh-miRn458 mimics, pmirGLO-WRI1-MUT+mimics NC and pmirGLO-WRI1-MUT+hrh-miRn458 mimics, respectively.). *** Significant difference at the level of P<0.001; ns means no significant difference (P>0.05).