基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用
何晓玲, 刘鹏程, 马伯军, 陈析丰(
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Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants
He Xiaoling, Liu Pengcheng, Ma Bojun, Chen Xifeng()
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图7. 基因组修饰系统去甲基化和甲基化的工作原理
(A) DNA去甲基化修饰, dCas9-SunTag系统招募人脱甲基酶TET1cd (棕色), 在目的基因的启动子区使DNA去甲基化, Me表示甲基化; (B) DNA甲基化修饰, dCas9-SunTag系统招募烟草DRM甲基转移酶催化结构域NtDRMcd (绿色), 在目的基因启动子区使DNA甲基化。PAM: 原间隔序列邻近基序
Figure 7. Technical principles of demethylation and methylation of genome modification systems
(A) DNA demethylation modification, dCas9-SunTag system recruits human demethylase TET1cd (brown) to demethylate DNA in the promoter region of the target gene, Me represents methylation; (B) DNA methylation modification, dCas9-SunTag system recruits tobacco DRM methyltransferase catalytic domain NtDRMcd (green) to methylate DNA in the promoter region of the target gene. PAM: Protospacer adjacent motif
