基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用
何晓玲, 刘鹏程, 马伯军, 陈析丰(
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Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants
He Xiaoling, Liu Pengcheng, Ma Bojun, Chen Xifeng()
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图6. CRISPR激活系统的工作原理
(A) 转录激活子激活目的基因转录, dCas9与转录激活子(洋红色)融合, 招募RNA聚合酶(RNAP)并激活目的基因的转录; (B)-(E) 不同CRISPRa系统示意图, 包括TALs (橙红色)、VP64 (绿色)、MS2 (浅黄色)、MCP (深黄色)、GCN4 (湛蓝色)和scFv (明黄色)。PAM: 原间隔序列邻近基序
Figure 6. Technical principles of CRISPR activation system
(A) Transcriptional activator activates the transcription of the target gene, dCas9 fuses with transcription activator (magenta) to recruit RNA polymerase (RNAP) and activates the transcription of the target gene; (B)-(E) Schematic diagram of different CRISPRa systems, including TALs (orange red), VP64 (green), MS2 (light yellow), MCP (deep yellow), GCN4 (azure blue) and scFv (bright yellow). PAM: Protospacer adjacent motif
