基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用
何晓玲, 刘鹏程, 马伯军, 陈析丰(
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Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants
He Xiaoling, Liu Pengcheng, Ma Bojun, Chen Xifeng()
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图4. 多核苷酸靶向删除系统(AFID)的工作原理
Cas9切割双链产生双链断裂(DSB), 尿嘧啶-DNA-糖基化酶(UDG)将C脱氨生成的U水解为AP位点, 并借助AP裂合酶(橙红色)和核酸外切酶(棕色), 实现靶点C到DSB切口之间的多核苷酸删除。PAM: 原间隔序列邻近基序
Figure 4. Technical principles of APOBEC-Cas9 fusion-induced deletion systems (AFIDs)
Cas9 cuts both strands to form adouble strand break (DSB), uracil-DNA-glycosylase (UDG) hydrolyzes the U produced by C deamination to AP site, and achieve the polynucleotide deletion between target C and DSB with the help of AP lyase (orange red) and exonuclease (brown). PAM: Protospacer adjacent motif
