基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用

何晓玲, 刘鹏程, 马伯军, 陈析丰(

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Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants

He Xiaoling, Liu Pengcheng, Ma Bojun, Chen Xifeng(

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图3. 多重编辑系统(SWISS)的工作原理
(A), (B) SWISS系统使用不同的scRNAs (MS2和boxB), 招募融合了相应蛋白(MCP和N22p)的rAPOBEC1或ecTadA:ecTadA^*, 实现同时在不同位点的胞嘧啶碱基编辑(CBE)和腺嘌呤碱基编辑(ABE); (C) 使用1对sgRNA在第3个靶点产生DNA双链断裂(DSB), 诱导细胞进行同源定向修复(HDR), 产生随机突变。PAM: 原间隔序列邻近基序; UGI: 尿嘧啶糖基化酶抑制剂

Figure 3. Technical principles of simultaneous and wide-editing induced by a single system (SWISS)
(A), (B) Mediated by different scRNAs (MS2 and boxB), SWISS recruits rAPOBEC1 or ecTadA:ecTadA^* that combines the corresponding proteins (MCP and N22p) to achieve cytidine (CBE) and adenine (ABE) base editing at different sites; (C) Mediated by a pair of sgRNAs, Cas9 produces double strand break (DSB) at the third target, induces homology-directed repair (HDR) and produces random mutation. PAM: Protospacer adjacent motif; UGI: Uracil glycosylase inhibitor