基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用
何晓玲, 刘鹏程, 马伯军, 陈析丰(
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Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants
He Xiaoling, Liu Pengcheng, Ma Bojun, Chen Xifeng()
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图2. 先导编辑器(PE)的工作原理
在nCas9H840A (绿)和逆转录酶M-MLV (黄)的作用下, 系统PE将碱基序列连接到靶位点, 借助DNA修复实现任意碱基的转换以及小片段的插入或删除。PAM: 原间隔序列邻近基序; PBS: 引物结合位点
Figure 2. Technical principles of prime editor (PE)
Mediated by nCas9H840A (green) and reverse transcriptase M-MLV (yellow), PE installs the base sequence into the target site, and achieves arbitrary base conversion and precise insertion and deletion of small fragments after DNA repair. PAM: Protospacer adjacent motif; PBS: Primer binding site
