铁观音原生质体高效瞬时转化方法的建立
张玉琴1,2, 吴嘉诚1,2, 何萌2, 刘仁义3, 朱晓玥2,*(
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An Efficient Protoplast Transient Expression System in Camellia sinensis var. sinensis cv. ‘Tieguanyin’
Yuqin Zhang1,2, Jiacheng Wu1,2, Meng He2, Renyi Liu3, Xiaoyue Zhu2,*()
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图4. 拟南芥和铁观音叶肉原生质体中GSII-1.1和TSI蛋白亚细胞定位
(A)-(D) 拟南芥原生质体中转化HBT-GFP-GSII-1.1; (E)-(H) 拟南芥原生质体中转化HBT-GFP-TSI; (I)-(L) 铁观音叶肉原生质体中转化HBT-GFP-GSII-1.1; (M)-(P) 铁观音叶肉原生质体中转化HBT-GFP-TSI。Bars=10 μm
Figure 4. GSII-1.1 and TSI subcellular localization in Arabidopsis thaliana and Tieguanyin mesophyll protoplasts
(A)-(D) Transformation of HBT-GFP-GSII-1.1 in Arabidopsis thaliana protoplasts; (E)-(H) Transformation of HBT-GFP-TSI in Arabidopsis thaliana protoplasts; (I)-(L) Transformation of HBT-GFP-GSII-1.1 in Tieguanyin protoplasts; (M)-(P) Transformation of HBT-GFP-TSI in Tieguanyin protoplasts. Bars=10 μm
