大花银莲花愈伤组织诱导及再生体系的建立

逯锦春¹, 曹丽娜¹, 佟冠杰², 王鑫颖¹, 张利英¹, 喻锌¹, 李荟芳¹, 李彦慧¹^,^*(

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Establishment of Callus Induction and Regeneration System of Anemone silvestris

Jinchun Lu¹, Lina Cao¹, Guanjie Tong², Xinying Wang¹, Liying Zhang¹, Xin Yu¹, Huifang Li¹, Yanhui Li¹^,^*(

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图1. 大花银莲花再生体系的建立
(A) 无菌苗; (B) 上胚轴诱导的不定芽; (C) 下胚轴诱导出的愈伤组织及不定芽; (D) 叶片诱导出的愈伤组织; (E) 叶片愈伤组织分化出的不定芽; (F) 叶柄诱导出的愈伤组织及分化的不定芽; (G) 不定芽的增殖; (H) 不定芽生根; (I) 移栽。Bars=2 cm

Figure 1. Establishment of regeneration system of Anemone silvestris
(A) Sterile seedling; (B) Adventitious buds induced by epicotyl; (C) Hypocotyl-induced callus and adventitious buds; (D) Callus induced from leaves; (E) Adventitious buds differentiated from leaf callus; (F) Callus induced by petiole and differentiated adventitious buds; (G) Adventitious buds multiplication; (H) Adventitious bud rooting; (I) Transplanting. Bars=2 cm