MBF1调控植物热应答与生长发育分子机制研究进展

秦怡¹, 刘艳爽¹^,², 仇柳柳¹, 周敏¹, 杜小杉¹, 戴绍军¹^,^*(

), 孙美红¹^,^*(

)

Advance in Molecular Mechanism of MBF1 Regulating Plant Heat Response and Development

Yi Qin¹, Yanshuang Liu¹^,², Liuliu Qiu¹, Min Zhou¹, Xiaoshan Du¹, Shaojun Dai¹^,^*(

), Meihong Sun¹^,^*(

)

图1. 植物MBF1系统发育关系、基因结构和保守基序分析
(A) 植物MBF1的系统发育关系、基因结构和保守基序分布图。11个物种MBF1蛋白的全长蛋白质序列来自JGI数据库(https:// phytozome-next.jgi.doe.gov/) (表1)。用MEGA7.0软件基于邻接法构建同源系统进化树, 绿色背景表示I类MBF1蛋白亚家族, 红色背景表示II类MBF1蛋白亚家族。利用GSDS2.0软件(http://gsds.cbi.pku.edu.cn/)在线绘制基因结构图, 上游5'非编码区/下游3'非编码区用蓝色方框表示, 外显子用黄色方框表示, 内含子用黑色直线显示。使用MEME网站(https://meme-suite.org/meme/ tools/meme)在线预测保守基序。通过NCBI网站CDD数据库(https://www.ncbi.nlm.nih.gov/cdd)鉴定蛋白质序列的保守结构域及功能。利用Tbtools软件绘制保守基序分布图, 5个保守基序用不同颜色方框表示。(B) 11个物种MBF1蛋白中预测到的保守基序序列。

Figure 1. The analysis of the phylogenetic relationships, gene structures and conserved motifs of plant MBF1
(A) Phylogenetic relationship, gene structure and conserved motif distribution map of plant MBF1. The full-length protein sequences of MBF1 proteins from 11 species are downloaded from the JGI database (https://phytozome-next.jgi.doe.gov/) (table 1). MEGA7.0 software is used to construct a Neighbor-Joining homologous phylogenetic tree. The green background indicates the type I subfamily, and the red background indicates the type II subfamily. GSDS2.0 software (http://gsds.cbi.pku.edu.cn/) is used to draw a gene structure diagram online. 5'UTR/3'UTR are represented by blue boxes, exons are represented by yellow boxes, and introns are represented by black straight lines. The MEME website (https://meme-suite.org/meme/tools/meme) is used to predict conserved motifs. Conserved domains and functions are identified through the NCBI website CDD database (https://www.ncbi.nlm.nih.gov/cdd). Tbtools software is used to draw a map of the conserved motifs, and the 5 conserved motifs are indicated by boxes with different colors. (B) The predicted conserved motif sequences in MBF1 proteins of 11 species.