毛报春(Primula × pubescens)腋芽再生组织培养体系的建立

李孟悦, 刘柳, 刘艳, 张晓曼

Establishment of Tissue Culture System for Axillary Bud Regeneration of Primula × pubescens

Mengyue Li, Liu Liu, Yan Liu, Xiaoman Zhang

表2 不同浓度NAA与6-BA配比对毛报春不定芽增殖诱导的影响

Table 2 Effect of NAA and 6-BA ratio at different concentrations on Primula × pubescens adventitious bud proliferation

No. Culture medium Inoculation number Multiplication rate (%) Growth situation 1 1/2MS+0.2 mg∙L-1 NAA+0.2 mg∙L-1 6-BA 30 0 - 2 1/2MS+0.2 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 3.33±3.33 cd + 3 1/2MS+0.2 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 6.67±6.67 cd + 4 1/2MS+0.2 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 13.33±3.33 bcd + 5 1/2MS+0.5 mg∙L-1 NAA+0.2 mg∙L-1 6-BA 30 20.00±11.55 bcd + 6 1/2MS+0.5 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 34.67±11.78 abcd + 7 1/2MS+0.5 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 10.00±10.00 bcd + 8 1/2MS+0.5 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 0 - 9 1/2MS+1.0 mg∙L-1 NAA+0.2 mg∙L-1 6-BA 30 67.00±11.36 a ++ 10 1/2MS+1.0 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 26.67±3.33 bcd + 11 1/2MS+1.0 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 23.33±3.33 bcd + 12 1/2MS+1.0 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 30.00±0.00 abcd + 13 MS+0.2 mg∙L-1 NAA+0.2 mg∙L-1 6-BA 30 40.00±11.55 abc +++ 14 MS+0.2 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 3.33±3.33 cd + 15 MS+0.2 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 16.67±3.33 bcd ++ 16 MS+0.2 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 38.00±1.15 abc ++ 17 MS+0.5 mg∙L-1 NAA+0.2 mg∙L-1 6-BA 30 67.00±11.35 a ++++ 18 MS+0.5 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 47.33±6.36 ab +++ 19 MS+0.5 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 0 - 20 MS+0.5 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 0 - 21 MS+1.0 mg∙L-1 NAA+0.2 mg∙L-1 6-BA 30 6.67±6.67 cd + 22 MS+1.0 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 17.00±8.89 bcd ++ 23 MS+1.0 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 6.67±6.67 cd + 24 MS+1.0 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 13.33±8.82 bcd ++