毛报春(Primula × pubescens)腋芽再生组织培养体系的建立

李孟悦, 刘柳, 刘艳, 张晓曼

Establishment of Tissue Culture System for Axillary Bud Regeneration of Primula × pubescens

Mengyue Li, Liu Liu, Yan Liu, Xiaoman Zhang

表1 不同浓度NAA与6-BA配比对毛报春愈伤组织诱导及分化的影响

Table 1 Effect of NAA and 6-BA ratio at different concentrations on Primula × pubescens callus induction and differentiation

No. Culture medium Inoculation number Callus induction rate (%) Callus growth situation Callus differentiation rate (%) Cluster bud growth situation 1 1/2MS+0.1 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 6.67±3.33 fg + 0 - 2 1/2MS+0.1 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 15.67±2.96 efg + 3.33±3.33 cd + 3 1/2MS+0.1 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 6.67±6.67 fg + 6.67±6.67 cd + 4 1/2MS+0.2 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 63.33±8.81 abcd ++ 13.33±3.33 bcd + 5 1/2MS+0.2 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 43.33±12.02 bcde + 20.00±11.55 bcd + 6 1/2MS+0.2 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 76.33±8.21 ab ++ 34.70±11.78 abcd + 7 1/2MS+0.5 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 0 - 10.00±10.00 bcd + 8 1/2MS+0.5 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 40.00±5.77 cdef + 0 - 9 1/2MS+0.5 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 6.00±3.05 fg + 67.00±11.36 a ++ 10 1/2MS+1.0 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 6.67±3.33 fg + 26.70±3.33 bcd + 11 1/2MS+1.0 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 0 - 23.33±3.33 bcd + 12 1/2MS+1.0 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 0 - 30.00±30.00 abcd ++ 13 MS+0.1 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 83.33±8.82 a ++++ 40.00±11.55 abc +++ 14 MS+0.1 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 76.67±6.67 ab ++++ 3.33±3.33 cd + 15 MS+0.1 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 50.00±11.55 abcde +++ 16.70±3.33 bcd + 16 MS+0.2 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 70.67±1.20 abc ++++ 38.70±1.15 abc ++ 17 MS+0.2 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 84.00±11.37 a ++++ 67.00±11.36 a ++++ 18 MS+0.2 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 38.33±7.27 cdef ++ 47.30±6.36 ab +++ 19 MS+0.5 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 70.00±5.77 abc ++++ 0 - 20 MS+0.5 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 63.33±8.82 abcd +++ 0 - 21 MS+0.5 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 16.00±3.06 efg + 6.67±6.67 cd + 22 MS+1.0 mg∙L-1 NAA+0.5 mg∙L-1 6-BA 30 32.00±2.00 defg ++ 17.00±8.89 bcd + 23 MS+1.0 mg∙L-1 NAA+1.0 mg∙L-1 6-BA 30 0 - 6.67±6.67 cd + 24 MS+1.0 mg∙L-1 NAA+2.0 mg∙L-1 6-BA 30 0 - 13.33±8.82 bcd +