图4. 拟南芥质子动力势(pmf)及其组分(ΔΨ和ΔpH)的测定
拟南芥离体叶片在光强为100 μmol·m^-2·s^-1 (WT 100, 黑)和750 μmol·m^-2·s^-1 (WT 750, 红)的活化光(635 nm)下照光3分钟, 照光期间提供稳定的CO₂ (380 μmol·mol^-1)、湿度(60%大气湿度)和温度(23°C)。黑暗时间60秒, 曲线为3次重复的平均值。测量前, 拟南芥叶片暗适应至少3小时。向上箭头表示活化光打开, 向下箭头表示活化光关闭。

Figure 4. Detection the proton motive force (pmf) and its two components (Δψ and ΔpH) in Arabidopsis thaliana
The detached leaf of Arabidopsis thaliana was exposed to light (635 nm, 100 μmol·m^-2·s^-1, labeled as WT 100, black and 750 μmol·m^-2·s^-1, labeled as WT 750, red) for 3 min, and provided stable CO₂ (380 μmol·mol^-1), humidity (60% atmospheric humidity) and temperature (23°C) during illumination. The dark time was 60 s and the curve corresponded to 3 independent biological replicates. The Arabidopsis leaf was darkly adapted for at least 3 hours before measurement. The up arrow indicated light on, and the down arrow indicated light off.