图3. 测量莱茵衣藻围绕PSI的环式电子传递速率
莱茵衣藻野生型(WT)和PGR5突变体(Mutant)在光强为80 μmol·m^-2·s^-1的活化光(635 nm)下照光5分钟, 关光后检测围绕PSI的环式电子传递速率, 黑暗持续时间为250 ms。测量前, 藻细胞(浓度约25 μg·mL^-1)加入10 µmol·L^-1二氯苯基二甲脲(DCMU)、1 mmol·L^-1羟胺(HA)和20%的聚蔗糖, 暗适应20分钟。箭头表示活化光关闭。

Figure 3. Detection the cyclic electron flow rate around photosystem I in Chlamydomonas reinhardtii
The C. reinhardtii cells (WT) and PGR5 mutant (Mutant) were exposed to light (635 nm, 80 μmol·m^-2·s^-1) for 5 min. The cyclic electron flow rate was detected after the light was turned off and the darkness duration was 250 ms. Before measurement, algal cells (about 25 μg·mL^-1) were incubated with 10 μmol·L^-1 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), 1 mmol·L^-1 hydroxylamine (HA) and 20% ficoll for 20 min in darkness. The arrow indicated light off.