图2. 测量拟南芥叶绿体ATP合酶的质子传导性
拟南芥离体叶片在光强为500 μmol·m^-2·s^-1的活化光(635 nm)下照光10分钟, 照光期间提供稳定的CO₂ (380 μmol·mol^-1)、湿度(60%大气湿度)和温度(23°C)。检测黑暗250 ms的P515衰减动力学, 重复100次后取平均值。ATP合酶的质子传导率为P515衰减动力学曲线半衰期(t_1/2)的倒数, 即94.3·s^-1。箭头表示活化光关闭。

Figure 2. Detection the H⁺ conductivity of chloroplast ATP synthase in Arabidopsis thaliana
The detached leaf of Arabidopsis thaliana was exposed to light (635 nm, 500 μmol·m^-2·s^-1) for 10 min, and provided stable CO₂ (380 μmol·mol^-1), humidity (60% atmospheric humidity) and temperature (23°C) during illumination. Detected the P515 decay kinetics curve in darkness that lasted 250 ms, the curve corresponded to 100 independent biological replicates. The H⁺ conductivity of ATP synthase was 94.3·s^-1, which is the reciprocal of the half-time (t_1/2) of P515 decay kinetics curve. The arrow indicated light off.