图1. 饱和闪光诱导的P515快速动力学
(A) 饱和闪光诱导的P515快速动力学示意图, 包括快速上升阶段(橙)、缓慢上升阶段(蓝)和缓慢下降阶段(绿), 箭头表示1次单周转饱和闪光(ST); (B) 饱和闪光诱导莱茵衣藻在520-550 nm的快速吸收变化(单周转饱和闪光波长635 nm, 光强200000 μmol·m^-2·s^-1, 持续时间5 μs), 莱茵衣藻野生型(WT) (浓度约25 μg·mL^-1)加入(红)和不加入(黑) 10 µmol·L^-1二氯苯基二甲脲(DCMU)和1 mmol·L^-1羟胺(HA), 测量前莱茵衣藻暗适应20分钟; (C) 预照光对P515快速动力学的影响, 莱茵衣藻野生型在暗适应20分钟(黑)以及在光下持续照光2分钟(光强40 μmol·m^-2·s^-1), 黑暗1分钟(红)后的P515快速动力学。

Figure 1. The P515 fast kinetics induced by a single turnover saturating flash
(A) The diagram of P515 fast kinetics induced by a single turnover saturating flash, rapid ascent stage (orange), slow ascent stage (blue) and slow descent stage (green), the arrow indicated a single turnover saturating flash (ST); (B) The rapid absorption changes of Chlamydomonas reinhardtii cells (WT) at 520-550 nm induced by a ST (the wavelength of ST was 635 nm, the light intensity was 200000 μmol·m^-2·s^-1 and the duration was 5 μs), WT (concentration 25 μg·mL^-1) was incubated with (red) and without (black) 10 μmol·L^-1 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 1 mmol·L^-1 hydroxylamine (HA) for 20 min in darkness; (C) The affection of preillumination to the P515 fast kinetics, WT algae was incubated in darkness for 20 min (black) and preilluminated for 2 min at 40 μmol·m^-2·s^-1 followed by 1 min dark (red).