图3. 生物素标记蛋白的免疫印迹检测
(A) 对野生型(WT)、转TurboID-EYFP (TY/WT)和转TurboID-EYFP-ATG8a (TY-8A/WT)拟南芥幼苗分别进行生物素处理(+, 50 µmol∙L^-1, 3小时)或不进行处理(-), 使用SA-HRP与anti-GFP抗体进行免疫印迹检测, 分析TurboID-EYFP的活性与表达水平; (B) 对不同株系的转TurboID-EYFP (TY/WT)拟南芥幼苗进行生物素处理(+, 50 µmol∙L^-1, 3小时)或不进行处理(-), 进行免疫印迹检测。SA-HRP: 辣根过氧化物酶标记链霉亲和素; CBB: 考马斯亮蓝

Figure 3. Immunoblot analysis of biotinylated protein
(A) Wild-type (WT), transgenic Arabidopsis seedlings overexpressing TurboID-EYFP (TY/WT) or TurboID-EYFP-ATG8a (TY-8A/WT) were treated with 50 µmol∙L^-1 biotin (+) for 3 h or 0 (-), activity and expression of TurboID-EYFP construct were analyzed by immunoblots with SA-HRP and anti-GFP antibodies; (B) Different lines of transgenic Arabidopsis seedlings overexpressing TurboID-EYFP (TY/WT) were treated with 50 µmol∙L^-1 biotin (+) for 3 h or 0 (-), followed by immunoblots. SA-HRP: Streptavidin-HRP; CBB: Coomassie Brilliant Blue