基于TurboID的植物蛋白邻近标记实验方法
邝嘉怡, 李洪清, 沈文锦*(
), 高彩吉*()
), 高彩吉*()
Methods for TurboID-based Proximal Labeling in Plants
Jiayi Kuang, Hongqing Li, Wenjin Shen*(), Caiji Gao*()
), Caiji Gao*()
图2. 稳定转化拟南芥中TurboID-EYFP-ATG8a的亚细胞定位
将6日龄转TurboID-EYFP-ATG8a拟南芥幼苗转移至含1 µmol∙L-1 Conc A的MS缺氮液体培养基(MS-N)处理8小时, 在激光共聚焦荧光显微镜下观察亚细胞定位。Bars=50 μm
Figure 2. Subcellular localization of TurboID-EYFP-ATG8a in stable Arabidopsis lines
6-day-old transgenic Arabidopsis seedlings overexpressing TurboID-EYFP-ATG8a were transferred to nitrogen (N)- deficient medium with 1 µmol∙L-1 Conc A for 8 h followed by confocal microscopic analysis. Bars=50 μm
