濒危植物百山祖冷杉种子发育特征及其胚培养
刘柯1, 刘彬1, 袁璐1, 帅辉1, 杨洋1, 王挺进1, 陈德良2, 陈小荣2, 杨锴斌2, 金孝锋3, 陈利萍1,*()
Seed Development and Embryo Culture of Endangered Abies beshanzuensis
Ke Liu1, Bin Liu1, Lu Yuan1, Hui Shuai1, Yang Yang1, Tingjin Wang1, Deliang Chen2, Xiaorong Chen2, Kaibin Yang2, Xiaofeng Jin3, Liping Chen1,*()

图5. 百山祖冷杉种子及其胚与胚乳发育过程的形态结构
(A) 5月25日的胚珠; (B) 6月25日类型I种子的胚与胚乳; (C) 6月25日类型II种子的胚乳; (D) 5月25日胚珠的石蜡切片, 红色方框内为花粉(上)与颈卵器(下); (E) 6月25日类型I种子的石蜡切片, 红色方框内为胚; (F) 6月25日类型II种子的石蜡切片; (G) 7月25日类型I种子的胚与胚乳; (H) 7月25日类型II种子的胚乳; (I) 7月25日类型III种子内皱缩的胚乳或/和胚; (J) 7月25日类型I种子的石蜡切片, 红色方框内为胚; (K) 7月25日类型II种子的石蜡切片; (L) 7月25日类型III种子的石蜡切片; (M) 8月25日类型I种子的胚与胚乳; (N) 8月25日类型III种子; (O) 9月25日类型I种子的胚与胚乳。Bars=1 000 μm。MF: 珠孔; In: 珠被; Em: 胚; Es: 胚乳; Po: 花粉; FG: 雌配子体; Re: 树脂囊; Ext: 外种皮; Ent: 内种皮; Cy: 子叶; Hy: 下胚轴; Su: 胚柄系统; ST: 皱缩组织; ER: 胚根; CC: 腐蚀腔

Figure 5. Morphology of Abies beshanzuensis seed and its embryo and endosperm during development
(A) The ovule on May 25th; (B) The embryo and endosperm of type I seeds on June 25th; (C) The endosperm of type II seeds on June 25th; (D) The paraffin section of the ovule on May 25th, the pollen (top) and archegonium (bottom) are in the red box; (E) The paraffin section of type I seeds on June 25th, the embryo in the red box; (F) The paraffin section of type II seeds on June 25th; (G) The embryo and endosperm of type I seeds on July 25th; (H) The endosperm of type II seeds on July 25th; (I) The shriveled tissue of endosperm or/and embryo within type III seeds on July 25th; (J) The paraffin section of type I seeds on July 25th, the embryo in the red box; (K) The paraffin section of type II seeds on July 25th; (L) The paraffin section of type III seeds on July 25th; (M) The embryo and endosperm of type I seeds on August 25th; (N) Type III seeds on August 25th; (O) The embryo and endosperm of type I seeds on September 25th. Bars=1 000 μm. MF: Micropylar funnel; In: Integument; Em: Embryo; Es: Endosperm; Po: Pollen; FG: Female gametophyte; Re: Resinocyst; Ext: Exotesta; Ent: Endotesta; Cy: Cotyledon; Hy: Hypocotyl; Su: Suspensor system; ST: Shriveled tissue; ER: Embryo root; CC: Corrosion cavity