小桐子半胱氨酸蛋白酶家族和相应miRNAs的鉴定及其对低温锻炼的响应

吴丹丹, 陈永坤, 杨宇, 孔春艳, 龚明

Identification of the Cysteine Protease Family and Corresponding miRNAs in Jatropha curcas and Their Response to Chill-hardening

Dandan Wu, Yongkun Chen, Yu Yang, Chunyan Kong, Ming Gong

表1 qRT-PCR相关引物

Table 1 The related primers for qRT-PCR

Primer name Sequence (5′→ 3′) miR1314-y-F GCCGGTCTCCAATGTTAGG miR1151-y-F ATCTGGTTGTGGGACCCG miR5156-x-F GCGAGACTGTGAAACTGCAAA miR6483-y-F GCGTTGTAGAAATTTTCAGGATCA JcXBCP3L-F TCTGTGGGTATGGATGGTTCTGC JcXBCP3L-R ACCCCAGTCAGTACCCCACGA JcRD21B-F CAACGCTTTAGGAGAGAAGGAA JcRD21B-R ACGCAACTTGTTCCTCCTGAC JcDEK1-F TGCTGGGAAATTCTGGTG JcDEK1-R AGCCGTCAAACCCACCA Reverse primer GCTGTCAACGATACGCTACGTAACG U6-F GGAACGATACAGAGAAGATT GAPDH-F TGAAGGACTGGAGAGGTGGAAGAGC GAPDH-R ATCAACAGTTGGAACACGGAAAGCC