植物小RNA荧光原位杂交实验方法
胡滨滨1,2, 薛治慧1, 张翠1,2,*(
)
)
Protocols for Small RNA FISH in Plants
Binbin Hu1,2, Zhihui Xue1, Cui Zhang1,2,*()
)
图1. 小RNA荧光原位杂交实验流程图
(A) 探针设计; (B) 石蜡切片; (C) 探针杂交; (D) 一抗孵育; (E) 二抗孵育; (F) 光谱拆分以及激光共聚焦显微成像
Figure 1. Outlines of small RNA fluorescent in situ hybridization
(A) Probe design; (B) Paraffin sectioning; (C) Probe hybridization; (D) Primary antibody incubation; (E) Secondary antibody incubation; (F) Spectral unmixing and imaging by confocal microscope
