外源海藻糖增强高表达转玉米C4型PEPC水稻耐旱性的机制

李佳馨, 李霞, 谢寅峰

Mechanism on Drought Tolerance Enhanced by Exogenous Trehalose in C4-PEPC Rice

Jiaxin Li, Xia Li, Yinfeng Xie

表1 qRT-PCR的基因和引物

Table 1 Genes and primers for qRT-PCR

Gene Forward primer (5′-3′) Reverse primer (5′-3′) Act CCCTCTTTCATCGGTATGGA TTGATCTTCATGCTGCTTGG OsTPP1 CAAATGGATTTGAGCAATAGC TCACACTGAGTGCTTCTTCCA OsTPP2 ATGGATTTGAAGACAAGCAAC TTAAGTGGATTCCTCCTTCCA OsTPP3 ATGACGAACCACGCCGGC CTACTTGCCAATCAGCCCTTT OsTPP7 CCTTCATGAGCGAGACGATG TCACGAACTCGAACACCTTG OsTre1 TTGGTACCCCTTACTCCGGCCGATTCA AAGAGCTCCTGCCTAGCCTAGCCACAT OsTPS1 AGTTATTATCTGGAAGGAGC TCAAGAACCTCCTGAATGCC OsTPS2 ACAAAGATGGGATGAAAGTG CAGGATTCACAAACAGATTC OsTPS8 TGAAGAGATAAAATGGCGTG GAAAAGGTGAATGAATCTGC OsSnR1a AACCAGAGGTAACAGGCAGG CATCTGTCAAGGAATGCAGG OsSnRK24 CGTGTTGGCTTCAGTGAAT CCTTCTCTATCTAAGGGCCG OsSAPK8 ATAGATGATAATGTCCAGCGTGAG GTTCCTACAGTGGATTTTGGTTG OsSAPK9 CACAGCAACGCCGTCTCC CACACTTCCACCGCTACCAA OsSAPK10 TGCTGATGTGTGGTCGTGTG TGCTGGTATGGTCGCCTCT OsABA8ox2 CTACTGCTGATGGTGGCTGA CCCATGGCCTTTGCTTTAT OsABA8ox3 AGTACAGCCCATTCCCTGTG ACGCCTAATCAAACCATTGC OsNCED3 CCCCTCCCAAACCATCCAAACCGA TGTGAGCATATCCTGGCGTCGTGA OsNCED4 TCCATCTCCTTCTCCCTCCTCCCA CCTCGCACCCTGCTTGATCTTGCC OsbZIP23 CTCTGATCCCTCGTTGCGTTA CAACACCCCAGCACCAAACT OsMYB2 GGGCTGAAACGCACAGGCAAGA GGGCTGAAACGCACAGGCAAGA OsRab16b CAACAACCACCAGCAGCA GATCTTGTCCATGAATCCC OsRab21 AGCAGCAGCATGCCATG TGGTGCCGGTGGTCAT OsLEA3 TTCCCACCAGGACCAGGCTA GTCGCCTCCTTGGTATCCT OsNAC6 CGCTGTACGAGAAGGAG ACTCGTGCATGATCCAGTTG C4-PEPC GTACCGCGAGTGGCCCGAGG CGTCCATGAGCTTGCGCCAC Osppc2a CAGCTACTCATGCTTAACGC GCACAGACTACAGCCTGTAC