水稻叶片水势的QTL定位与候选基因分析

潘晨阳, 张月, 林晗, 陈芊羽, 杨凯如, 姜嘉骥, 李梦佳, 芦涛, 王珂欣, 路梅, 王盛, 叶涵斐, 饶玉春, 胡海涛

QTL Mapping and Candidate Gene Analysis on Rice Leaf Water Potential

Chenyang Pan, Yue Zhang, Han Lin, Qianyu Chen, Kairu Yang, Jiaji Jiang, Mengjia Li, Tao Lu, Kexin Wang, Mei Lu, Sheng Wang, Hanfei Ye, Yuchun Rao, Haitao Hu

表1 实时荧光定量PCR的引物序列

Table 1 The primer sequences of real-time quantitative PCR

Primer name Sequence (5′-3′) Tm (°C) Length (bp) LOC_Os02g56630-F-qrt GATGCTACGGAGACTGCACA 60.02 182 LOC_Os02g56630-R-qrt GAGCCAGAAGCACATGAACA 59.99 LOC_Os02g57720-F-qrt CTGACGTCGTGGTCGTTCTA 59.90 101 LOC_Os02g57720-R-qrt GACTTGTTCACCCCCATCAC 60.22 LOC_Os04g43730-F-qrt ACCAAAGTGGGTGTTTCTCG 60.01 153 LOC_Os04g43730-R-qrt TCGAGATCTGGCTTGTGTTG 59.98 LOC_Os04g44060-F-qrt CGGTGTTCATGGTTCACTTG 60.00 182 LOC_Os04g44060-R-qrt CCTCAGGACGTACTGGTGGT 60.03 LOC_Os04g46490-F-qrt GCCTCGTCCTCCACTACATC 59.69 123 LOC_Os04g46490-R-qrt CCGTGTACACCACCATGAAC 59.73 LOC_Os02g57580-F-qrt ACCTCTTCAGATGGGGTGTG 59.96 101 LOC_Os02g57580-R-qrt CCAGTCAGTTTTGCAGACCA 59.87 LOC_Os04g48230-F-qrt GGGCACTACAAGTCCGTGAT 60.00 199 LOC_Os04g48230-R-qrt CTTGGTAGCTTCCGATGAGC 59.98 LOC_Os04g44570-F-qrt CGCCACCACTGGGTTTACT 60.96 123 LOC_Os04g44570-R-qrt CACGGGAAGCCGAGTATCT 60.23