蛋白质N-糖基化在拟南芥生长周期中的变化规律及去糖基化对根发育的影响
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王婷, 羊欢欢, 赵弘巍, JosefVoglmeir, 刘丽
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Changes of Protein N-glycosylation in the Growth of Arabidopsis thaliana and Effects of Enzymatic Deglycosylation on Root Development
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Ting Wang, Huanhuan Yang, Hongwei Zhao, Josef Voglmeir, Li Liu
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表3 不同时间点拟南芥各处理N-糖链相对含量
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Table 3 The relative content of each N-glycan of Arabidopsis from different groups at different time points
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| N-glycans | The relative content of each N-glycan (%) | | Blank | BSA0.05 | BSA0.5 | E0.05 | E0.5 | | Culture for 8 hours | | Man3XylFucGlcNAc2 | 35.22±0.42 b | 35.00±0.07 b | 35.57±0.99 b | 39.29±0.14 a | 40.25±0.62 a | | GlcNAcMan3XylFucGlcNAc2 | 18.12±0.07 bc | 17.10±0.07 b | 18.00±0.55 c | 18.51±0.05 b | 19.03±0.20 a | | Man4XylFucGlcNAc2 | 2.83±0.20 c | 2.61±0.10 c | 3.24±0.16 b | 3.63±0.14 a | 3.86±0.18 a | | GlcNAc2Man3XylFucGlcNAc2 | 9.98±0.13 c | 10.02±0.08 c | 10.95±0.79 b | 13.09±0.13 a | 12.89±0.55 a | | GalGlcNAcMan3XylFucGlcNAc2 | 2.63±0.09 c | 2.35±0.11 d | 2.35±0.16 d | 3.06±0.05 b | 3.83±0.12 a | | Man3XylGlcNAc2 | 6.98±0.16 c | 7.89±0.15 b | 7.86±0.19 b | 8.83±0.15 a | 6.66±0.32 c | | GlcNAcMan3XylGlcNAc2 | 2.77±0.14 a | 2.60±0.16 a | 2.95±0.22 a | 1.88±0.24 b | 1.94±0.21 b | | GlcNAc2Man3XylGlcNAc2 | 1.44±0.14 b | 1.69±0.10 a | 1.40±0.13 b | 1.67±0.21 ab | 1.64±0.11 ab | | Sum of complex | 79.97±0.52 c | 79.26±0.48 d | 82.31±0.22 b | 89.95±0.12 a | 90.10±0.29 a | | Man6GlcNAc2 | 4.78±0.10 a | 4.81±0.05 a | 3.41±0.27 b | 1.86±0.09 c | 1.70±0.16 c | | Man7GlcNAc2 | 4.16±0.13 a | 4.16±0.11 a | 3.25±0.11 b | 1.87±0.11 c | 1.58±0.29 c | | Man8GlcNAc2 | 5.37±0.10 a | 5.52±0.11 a | 4.90±0.20 b | 1.39±0.21 c | 1.44±0.18 c | | Man9GlcNAc2 | 5.71±0.28 b | 6.25±0.29 a | 6.12±0.13 a | 4.93±0.30 c | 5.18±0.13 c | | Sum of high-mannose | 20.03±0.52 b | 20.74±0.48 a | 17.69±0.22 c | 10.05±0.12 d | 9.90±0.29 d | | Culture for 5 days | | Man3XylFucGlcNAc2 | 29.60±0.18 b | 28.68±0.15 c | 28.60±0.87 c | 29.51±0.24 b | 30.74±0.36 a | | GlcNAcMan3XylFucGlcNAc2 | 17.97±0.10 b | 18.06±0.12 b | 18.15±0.61 b | 17.84±0.10 b | 19.85±0.28 a | | Man4XylFucGlcNAc2 | 4.46±0.08 a | 2.71±0.12 b | 2.24±0.15 c | 2.23±0.12 c | 2.34±0.11 c | | GlcNAc2Man3XylFucGlcNAc2 | 14.58±0.11 b | 13.80±0.15 c | 13.56±0.27 c | 13.54±0.18 c | 15.72±0.14 a | | GalGlcNAcMan3XylFucGlcNAc2 | 2.62±0.13 a | 2.29±0.10 b | 2.29±0.12 b | 2.46±0.11 ab | 2.22±0.18 b | | Man3XylGlcNAc2 | 5.17±0.07 d | 6.80±0.11 c | 8.56±0.26 a | 7.77±0.30 b | 5.00±0.16 d | | GlcNAcMan3XylGlcNAc2 | 1.81±0.17 d | 2.02±0.10 cd | 2.58±0.11 a | 2.33±0.11 b | 2.19±0.07 bc | | GlcNAc2Man3XylGlcNAc2 | 1.89±0.10 c | 3.14±0.05 a | 2.54±0.17 b | 3.36±0.25 a | 1.75±0.21 c | | Sum of complex | 78.10±0.10 c | 77.51±0.15 d | 78.52±0.26 c | 79.05±0.18 b | 79.80±0.52 a | | Man6GlcNAc2 | 5.46±0.09 c | 6.16±0.05 a | 5.64±0.15 bc | 5.81±0.30 ab | 5.38±0.32 c | | Man7GlcNAc2 | 6.09±0.13 a | 5.78±0.15 b | 4.90±0.13 d | 5.33±0.22 c | 4.87±0.11 d | | Man8GlcNAc2 | 6.22±0.15 b | 6.35±0.04 a | 6.41±0.09 a | 6.27±0.17 ab | 6.04±0.10 b | | Man9GlcNAc2 | 4.12±0.11 b | 4.20±0.15 ab | 4.53±0.26 a | 3.55±0.25 c | 3.90±0.20 bc | | Sum of high-mannose | 21.90±0.10 b | 22.49±0.15 a | 21.48±0.26 b | 20.95±0.18 c | 20.20±0.52 d | | Culture for 10 days | | Man3XylFucGlcNAc2 | 31.88±0.19 a | 31.64±0.09 ab | 31.58±0.08 ab | 29.30±0.12 c | 31.11±0.12 b | | GlcNAcMan3XylFucGlcNAc2 | 17.96±0.09 a | 17.66±0.10 a | 17.70±0.37 a | 17.07±0.09 b | 17.77±0.13 a | | Man4XylFucGlcNAc2 | 3.46±0.57 a | 3.57±0.11 a | 3.43±0.13 a | 3.32±0.12 a | 3.28±0.12 a | | GlcNAc2Man3XylFucGlcNAc2 | 14.34±0.07 ab | 13.88±0.10 bc | 14.50±0.58 a | 13.73±0.05 c | 14.85±0.16 a | | GalGlcNAcMan3XylFucGlcNAc2 | 2.05±0.09 a | 2.21±0.17 a | 2.30±0.20 a | 2.25±0.09 a | 2.20±0.23 a | | Man3XylGlcNAc2 | 5.81±0.23 c | 6.62±0.10 b | 5.48±0.38 c | 8.96±0.03 a | 5.86±0.14 c | | GlcNAcMan3XylGlcNAc2 | 2.20±0.15 a | 2.27±0.12 a | 2.51±0.42 a | 2.20±0.07 a | 2.19±0.11 a | | GlcNAc2Man3XylGlcNAc2 | 1.87±0.10 c | 2.23±0.13 b | 1.56±0.19 d | 2.64±0.07 a | 1.88±0.05 c | | Sum of complex | 79.57±0.47 ab | 80.08±0.30 a | 79.06±0.64 b | 79.47±0.07 ab | 79.15±0.15 b | | Man6GlcNAc2 | 5.47±0.09 a | 5.59±0.08 a | 5.39±0.32 a | 5.68±0.17 a | 5.52±0.10 a | | Man7GlcNAc2 | 5.36±0.16 b | 5.27±0.09 b | 5.24±0.10 b | 5.69±0.17 a | 5.66±0.17 a | | Man8GlcNAc2 | 6.20±0.07 a | 5.71±0.13 bc | 6.42±0.40 a | 5.52±0.21 c | 6.04±0.10 ab | | Man9GlcNAc2 | 3.41±0.22 b | 3.35±0.23 b | 3.89±0.15 a | 3.64±0.22 ab | 3.64±0.11 ab | | Sum of high-mannose | 20.43±0.47 ab | 19.92±0.30 b | 20.94±0.64 a | 20.53±0.07 a | 20.85±0.15 a |
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