蛋白质N-糖基化在拟南芥生长周期中的变化规律及去糖基化对根发育的影响
|
|
王婷, 羊欢欢, 赵弘巍, JosefVoglmeir, 刘丽
|
Changes of Protein N-glycosylation in the Growth of Arabidopsis thaliana and Effects of Enzymatic Deglycosylation on Root Development
|
|
Ting Wang, Huanhuan Yang, Hongwei Zhao, Josef Voglmeir, Li Liu
|
|
表2 拟南芥不同生长时期N-糖链中各类糖型的相对含量
|
Table 2 The relative content of different N-glycans from Arabidopsis thaliana at different growth stages
|
|
| N-glycans | Relative content (%) | | Seedling stage | Bolting stage | Flowering stage | Longhorn ripening stage | Aging stage | | Complex type with Xyl and Fuc | | Man3XylFucGlcNAc2 (MMXF) | 32.31±1.71 b | 24.79±0.33 c | 27.00±1.21 c | 37.96±1.92 a | 34.51±5.23 ab | | GlcNAcMan3XylFucGlcNAc2 (GnMXF) | 14.11±0.75 d | 20.18±1.05 b | 23.83±0.74 a | 17.37±1.67 c | 10.53±1.58 e | | Man4XylFucGlcNAc2 (M4XF) | 3.99±0.15 a | 3.11±0.36 ab | 2.70±0.40 b | 3.10±0.21 ab | 3.61±0.80 ab | | GlcNAc2Man3XylFucGlcNAc2 (GnGnXF) | 13.33±0.42 b | 15.68±0.15 b | 12.55±1.26 b | 16.06±1.03 b | 23.44±0.12 a | | GalGlcNAcMan3XylFucGlcNAc2 (GGnXF) | 4.69±0.36 a | 1.93±0.20 b | 1.61±0.29 b | 1.82±0.09 b | 1.58±0.36 b | | Sum | 68.44±1.51 b | 65.69±0.98 b | 67.68±2.33 b | 76.31±3.13 a | 73.84±2.79 a | | Complex type only with Xyl | | Man3XylGlcNAc2 (MMX) | 6.67±0.58 b | 6.62±0.23 b | 7.87±0.25 b | 11.59±1.68 a | 10.69±1.43 a | | GlcNAcMan3XylGlcNAc2 (GnMX) | 3.96±0.18 cd | 4.51±0.10 c | 3.77±0.08 d | 6.44±0.26 a | 5.46±0.65 b | | GlcNAc2Man3XylGlcNAc2 (GnGnX) | 7.06±0.82 a | 4.17±0.08 b | 2.71±0.79 bc | 3.30±0.62 bc | 3.80±0.41 c | | Sum | 17.69±1.57 b | 15.29±0.41 bc | 14.34±0.70 c | 21.33±2.39 a | 20.94±2.79 a | | Sum of complex | 86.13±2.95 b | 80.98±0.92 c | 82.02±1.65 c | 97.64±0.78 a | 94.77±0.94 a | | High-mannose type | | Man6GlcNAc2 (M6) | 5.85±0.32 a | 5.71±0.24 a | 6.31±0.22 a | 0.92±0.15 c | 2.77±0.77 b | | Man7GlcNAc2 (M7) | 3.63±0.69 b | 4.53±0.16 a | 4.41±0.30 ab | 0.39±0.30 c | 1.09±0.64 c | | Man8GlcNAc2 (M8) | 2.33±0.74 b | 4.72±0.29 a | 4.04±0.50 a | 0.20±0.09 c | 0.48±0.32 c | | Man9GlcNAc2 (M9) | 2.06±1.29 bc | 4.06±0.69 a | 3.23±0.67 ab | 0.84±0.83 c | 0.88±0.78 c | | Sum of high-mannose | 13.87±2.95 b | 19.02±0.92 a | 17.98±1.65 a | 2.36±0.78 c | 5.23±0.94 c |
|
|
|