蛋白质N-糖基化在拟南芥生长周期中的变化规律及去糖基化对根发育的影响

王婷, 羊欢欢, 赵弘巍, JosefVoglmeir, 刘丽

Changes of Protein N-glycosylation in the Growth of Arabidopsis thaliana and Effects of Enzymatic Deglycosylation on Root Development

Ting Wang, Huanhuan Yang, Hongwei Zhao, Josef Voglmeir, Li Liu

表1 拟南芥糖蛋白N-糖链结构

Table 1 The summary of N-glycan structures from glycoprotein in Arabidopsis thaliana

Number Retention time (min) GU value The theoretical electron charge-mass ratio (m/z) Detection value of charge-mass ratio (m/z) Name Abbreviation 1 17.2 4.86 1185.43 1185.51 Man3XylGlcNAc2 MMXF 2 17.9 5.03 - 806.99 Unknown - 3 19.4 5.39 1388.51 - GlcNAcMan3XylGlcNAc2 GnMXF 4 20.1 5.56 1331.49 1331.39 Man3XylFucGlcNAc2 MMX 5 21.4 5.90 1591.558 1591.74 GlcNAc2Man3XylGlcNAc2 GnGnX 6 22.3 6.10 1534.57 1534.70 GlcNAcMan3XylFucGlcNAc2 GnMX 7 23.2 6.35 1493.53 1494.16 Man4XylFucGlcNAc2 M4XF 8 24.0 6.58 1737.64 1737.93 GlcNAc2Man3XylFucGlcNAc2 GnGnXF 9 24.9 6.82 1696.62 1697.37 GalGlcNAcMan3XylFucGlcNAc2 GGnXF 10 25.5 6.97 1539.54 1540.16 Man6GlcNAc2 M6 11 27.3 7.47 - 1620.53 Unknown - 12 28.6 7.86 1701.89 1701.74 Man7GlcNAc2 M7 13 31.4 8.73 1863.65 1863.86 Man8GlcNAc2 M8 14 33.4 9.44 2025.70 2025.88 Man9GlcNAc2 M9