谷子茎尖体外遗传转化体系的建立与优化
杨澜, 刘雅, 项阳, 孙秀娟, 颜景畏, 张阿英*(
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Establishment and Optimization of a Shoot Tip-based Genetic Transformation System for Foxtail Millet
Lan Yang, Ya Liu, Yang Xiang, Xiujuan Sun, Jingwei Yan, Aying Zhang*()
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图2. 谷子转基因植株鉴定
(A) 谷子幼苗GUS染色图(bar=0.1 cm); (B) PCR鉴定(1: 阳性对照; 2: 阴性对照; 3: 切一刀的植株叶片; 4: 切两刀的植株叶片; 5: 不做切除处理的植株叶片)
Figure 2. Identification of transgenic foxtail millet
(A) Image from GUS staining of foxtail millet seedlings (bar=0.1 cm); (B) PCR identification (1: Positive control; 2: Negative control; 3: A leaf with one wound cut; 4: A leaf with two wounds cut; 5: A leaf without wound)
