谷子茎尖体外遗传转化体系的建立与优化
杨澜, 刘雅, 项阳, 孙秀娟, 颜景畏, 张阿英*(
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Establishment and Optimization of a Shoot Tip-based Genetic Transformation System for Foxtail Millet
Lan Yang, Ya Liu, Yang Xiang, Xiujuan Sun, Jingwei Yan, Aying Zhang*()
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图1. pCUN-NHF质粒T-DNA区段示意图
LB: LB T-DNA重复; Ubi promoter: 玉米Ubiquitin1启动子; HA: HA标签; MCS: 多克隆位点; 35S promoter: CaMV35S启动子; Bar: 抗草铵膦基因; RB: RB T-DNA重复
Figure 1. The T-DNA region of plasmid pCUN-NHF
LB: LB T-DNA repeat; Ubi promoter: Zea mays Ubiquitin1 promoter; HA: HA tag; MCS: Multiple cloning site; 35S promoter: CaMV35S promoter; Bar: Herbicide bialaphos-resistance gene; RB: RB T-DNA repeat
