谷子茎尖体外遗传转化体系的建立与优化

杨澜, 刘雅, 项阳, 孙秀娟, 颜景畏, 张阿英

Establishment and Optimization of a Shoot Tip-based Genetic Transformation System for Foxtail Millet

Lan Yang, Ya Liu, Yang Xiang, Xiujuan Sun, Jingwei Yan, Aying Zhang

表1 初步建立的体系和优化后的体系条件对比

Table 1 Comparison of the conditions of the initially established system and the optimized system

Agrobacterium concentration (OD600) Acetosyringone concentration (μmol?L-1) Pressure (MPa) Time (min) Before optimization 1.0 400 0.04 20 After optimization 1.4 800 0.05 40