基因枪介导的老芒麦遗传转化体系的建立
杜鹏飞1,2,3, 王玉2, 曹英萍2, 杨松2, 孙志超2, 毛德才3,4, 鄢家俊3,4, 李达旭3,4, 孙美贞5, 付春祥2,*(
), 白史且3,4,*()
), 白史且3,4,*()
Establishment of Biolistic Mediated Transformation System for Elymus sibiricus
Pengfei Du1,2,3, Yu Wang2, Yingping Cao2, Song Yang2, Zhichao Sun2, Decai Mao3,4, Jiajun Yan3,4, Daxu Li3,4, Meizhen Sun5, Chunxiang Fu2,*(), Shiqie Bai3,4,*()
), Shiqie Bai3,4,*()
图6. 不同诱导时间和预处理方式下的幼穗愈伤基因枪轰击后的荧光显微图
(A), (B) 25天幼穗愈伤(未进行轰击); (C), (D) 25天幼穗愈伤常规高渗处理; (E), (F) 25天幼穗愈伤滤纸干燥处理2小时; (G), (H) 35天的幼穗愈伤滤纸干燥处理2小时。箭头所示为阳性转化愈伤。Bars=20 μm
Figure 6. Fluorescence micrographs of inflorescence callus after bombardment with different induction time and pretreatment methods
(A), (B) Callus of inflorescence on 25 d (no bombardment); (C), (D) Conventional hyperosmotic treatment of inflorescence callus at 25 d; (E), (F) Inflorescence callus filter paper dried for 2 h on 25 d; (G), (H) Inflorescence callus filter paper dried for 2 h on 35 d. Arrows indicate the positive transgenic calli. Bars =20 μm
