基因枪介导的老芒麦遗传转化体系的建立

杜鹏飞¹^,²^,³, 王玉², 曹英萍², 杨松², 孙志超², 毛德才³^,⁴, 鄢家俊³^,⁴, 李达旭³^,⁴, 孙美贞⁵, 付春祥²^,^*(

), 白史且³^,⁴^,^*(

)

Establishment of Biolistic Mediated Transformation System for Elymus sibiricus

Pengfei Du¹^,²^,³, Yu Wang², Yingping Cao², Song Yang², Zhichao Sun², Decai Mao³^,⁴, Jiajun Yan³^,⁴, Daxu Li³^,⁴, Meizhen Sun⁵, Chunxiang Fu²^,^*(

), Shiqie Bai³^,⁴^,^*(

)

图5. 根癌农杆菌侵染川草2号老芒麦幼穗和幼穗愈伤
(A), (B) 农杆菌侵染幼穗愈伤GUS染色前后图片; (C), (D) 农杆菌侵染幼穗愈伤RFP荧光下明场和暗场图片; (E), (F) 农杆菌直接侵染幼穗后GUS染色前后图片; (G), (H) 农杆菌直接侵染幼穗后RFP下明场和暗场图片。Bars=50 μm

Figure 5. Callus of inflorescence and inflorescence infected by Agrobacterium tumefaciens in Elymus sibiricus cv. ‘Chuancao No.2’
(A), (B) Callus induced by inflorescence before and after GUS staining after Agrobacterium infection; (C), (D) The bright and dark field images of RFP fluorescence after Agrobacterium infection of callus induced by inflorescence; (E), (F) The images before and after GUS staining directly after Agrobacterium infection with inflorescence; (G), (H) The bright and dark field images of RFP fluorescence after Agrobacterium infection of inflorescence, respectively. Bars=50 μm