图4. 川草2号老芒麦组培再生体系建立及幼穗愈伤分化效率随继代时间的变化
(A) 幼穗接种于愈伤诱导培养基; (B) 幼穗诱导35天后的愈伤; (C) 挑选图(B)中结构致密坚硬的愈伤置于继代培养基15天; (D) 图(C)愈伤置于分化培养基约35天; (E) 图(D)中红色框内愈伤分化显微图; (F) 图(D)中分化苗置于生根培养基约30天; (G) 种子接种于愈伤诱导培养基; (H) 种子诱导35天后的愈伤; (I) 挑选图(H)中结构致密坚硬的愈伤置于继代培养基15天; (J) 图(I)愈伤置于分化培养基约35天; (K) 图(J)中红色框内愈伤分化显微图; (L) 不同再生时间下的幼穗愈伤分化效率(误差线为标准误, 样本容量为30, 不同小写字母表示差异显著(P<0.05))。(D), (F), (G), (J) Bars=2 cm; (A)-(C), (E), (H), (I), (K) Bars=1 cm

Figure 4. The process diagram of tissue culture and regeneration system and the plot of callus differentiation efficiency of Elymus sibiricus cv. ‘Chuancao No.2’ with subculture time
(A) Inflorescence of ‘Chuancao No.2’ placed in callus induction medium; (B) Callus induced by inflorescence of ‘Chuancao No.2’ for 35 d; (C) The callus with dense and hard structure in figure (B) was selected and placed in medium for 15 d; (D) Callus in figure (C) cultured in differentiation medium for 35 d; (E) The micrograph of callus in red frame in figure (D); (F) Differentiated seedlings in figure (D) placed in rooting medium for 30 d; (G) Seeds placed in callus induction medium; (H) Callus induced by seeds for 35 d; (I) The callus with dense and hard structure in figure (H) was selected and placed in subculture medium for 15 d; (J) Callus in figure (I) cultured in differentiation medium for 35 d; (K) The micrograph of callus in red frame in figure (J); (L) The differentiation efficiency of callus induced by inflorescence at different time periods (The error line is the standard error, and the sample size is 30; Different lowercase letters indicate significant differences at P<0.05). Bars in (D), (F), (G), (J)=2 cm; Bars in (A)-(C), (E), (H), (I), (K)=1 cm