基因枪介导的老芒麦遗传转化体系的建立
杜鹏飞1,2,3, 王玉2, 曹英萍2, 杨松2, 孙志超2, 毛德才3,4, 鄢家俊3,4, 李达旭3,4, 孙美贞5, 付春祥2,*(
), 白史且3,4,*()
), 白史且3,4,*()
Establishment of Biolistic Mediated Transformation System for Elymus sibiricus
Pengfei Du1,2,3, Yu Wang2, Yingping Cao2, Song Yang2, Zhichao Sun2, Decai Mao3,4, Jiajun Yan3,4, Daxu Li3,4, Meizhen Sun5, Chunxiang Fu2,*(), Shiqie Bai3,4,*()
), Shiqie Bai3,4,*()
图1. PANIC6A (A)、PANIC6D (B)和PANIC6E (C) T-DNA区段
LB: T-DNA区段左边界; OsAct1: 水稻启动子; hph/bar: 筛选标记基因; PvUbi1: 柳枝稷启动子; pporRFP/GUSplus: 红色荧光/葡萄糖苷酸酶报告基因; ZmUbi1: 玉米启动子; Cmr: 氯霉素抗性基因; ccdB: 目的基因; RB: T-DNA区段右边界
Figure 1. T-DNA segment of PANIC6A (A), PANIC6D (B) and PANIC6E (C)
LB: Left boundary of T-DNA segment; OsAct1: Rice promoter; hph/bar: Screening marker genes; PvUbi1: Switchgrass promoter; pporRFP/GUSplus: Red fluorescence/glucuronidase reporter gene; ZmUbi1: Maize promoter; Cmr: Chloramphenicol resistant gene; ccdB: Target gene; RB: Right boundary of T-DNA segment
