快速、无损大豆种子连续取样技术及其DNA制备
夏正俊1,*(), 李玉卓1,2, 朱金龙1, 吴红艳1, 徐坤1, 翟红1
A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed
Zhengjun Xia1,*(), Yuzhuo Li1,2, Jinlong Zhu1, Hongyan Wu1, Kun Xu1, Hong Zhai1

图4. 水稻、大豆和玉米种子大小及对应钻头型号
(A) 水稻种子(平均钻孔深度为(1.52±0.12) mm, 直径0.9 mm的钻头获取的平均组织重量为(1.54±0.12) mg, 直径1.1 mm的钻头获取的平均组织重量为(2.30±0.18) mg); (B) 小粒大豆种子(平均钻孔深度为(3.88±0.37) mm, 直径1.1 mm的钻头获取的平均组织重量为(5.85±0.99) mg, 直径1.2 mm的钻头获取的平均组织重量为(6.96±0.66) mg); (C) 大粒大豆种子(平均钻孔深度为(4.63±0.43) mm, 直径1.2 mm的钻头获取的平均组织重量为(8.31±0.79) mg, 直径1.3 mm的钻头获取的平均组织重量为(9.75±0.92) mg)。(D) 玉米种子(平均钻孔深度为(3.78±0.46) mm, 直径1.2 mm的钻头获取的平均组织重量为(6.79±0.83) mg, 直径1.3 mm的钻头获取的平均组织重量为(7.97±0.98) mg)。

Figure 4. The seed dimensions of rice, soybean and maize and the selection of their appropriate drillers
(A) Rice seed (average drilling depth is (1.52±0.12) mm; average acquired tissue weight is (1.54±0.12) mg for the 0.9 mm diameter driller; average acquired tissue weight is (2.30±0.18) mg for the 1.1 mm diameter driller). (B) Soybean seed (small size) (average drilling depth is (3.88±0.37) mm; average acquired tissue weight is (5.85±0.99) mg for the 1.1 mm diameter driller; average acquired tissue weight is (6.96±0.66) mg for the 1.2 mm diameter driller). (C) Soybean seed (large size) (average drilling depth is (4.63±0.43) mm; average acquired tissue weight is (8.31±0.79) mg for the 1.2 mm diameter driller; average acquired tissue weight is (9.75±0.92) mg for the 1.3 mm diameter driller). (D) Maize seed (average drilling depth is (3.78±0.46) mm; average acquired tissue weight is (6.79±0.83) mg for the 1.2 mm diameter driller; average acquired tissue weight is (7.97±0.98) mg for the 1.3 mm diameter driller.