快速、无损大豆种子连续取样技术及其DNA制备
夏正俊1,*(
), 李玉卓1,2, 朱金龙1, 吴红艳1, 徐坤1, 翟红1
), 李玉卓1,2, 朱金龙1, 吴红艳1, 徐坤1, 翟红1
A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed
Zhengjun Xia1,*(), Yuzhuo Li1,2, Jinlong Zhu1, Hongyan Wu1, Kun Xu1, Hong Zhai1
), Yuzhuo Li1,2, Jinlong Zhu1, Hongyan Wu1, Kun Xu1, Hong Zhai1
图3. 种子钻孔法鉴定结果的验证
钻孔的大豆种子出苗后, 提取其叶片DNA进行相关引物的基因型鉴定, 以验证种子基因型鉴定结果的准确性。上部和下部凝胶图分别为Satt557和S8的验证结果(底部标注了2个分子标记的基因型)。
Figure 3. Verification of genotyping results obtained through seed-drilling
DNA were extracted from leaves of the plants that were developed from the drilled seeds, thereafter genotyping of Satt557 and S8 were performed to verify the accuracy of genotyping data of seed. The upper and the bottom gels are shown the genotyping result of Satt557 and S8, respectively (the genotypes of each individual were indicated at the bottom of each lane).
