快速、无损大豆种子连续取样技术及其DNA制备
夏正俊1,*(
), 李玉卓1,2, 朱金龙1, 吴红艳1, 徐坤1, 翟红1
), 李玉卓1,2, 朱金龙1, 吴红艳1, 徐坤1, 翟红1
A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed
Zhengjun Xia1,*(), Yuzhuo Li1,2, Jinlong Zhu1, Hongyan Wu1, Kun Xu1, Hong Zhai1
), Yuzhuo Li1,2, Jinlong Zhu1, Hongyan Wu1, Kun Xu1, Hong Zhai1
图2. 大豆遗传群体经种子钻取及DNA制备后的基因型鉴定
利用Satt557和S8两对引物进行单管PCR能准确鉴定两分子标记间的重组个体(箭头)。M: 分子质量标准品ΦX174 HaeIII; 1-19: Harosoy-E1与Harosoy (e1)杂交的F2群体(在每个泳道底部标注有每个个体2个分子标记的基因型)。
Figure 2. Genotyping of a soybean genetic population through seed-drilling and thereafter DNA extraction
Accurate identification of recombinant occuring between two markers, Satt557 and S8, using one tube PCR (arrow). M: Molecular weight marker ΦX174 HaeIII; 1-19: F2 population that were derived from Harosoy-E1 × Harosoy (e1) (the genotypes of each individual were indicated at the bottom of each lane).
