快速、无损大豆种子连续取样技术及其DNA制备
|
夏正俊 1,*( ), 李玉卓 1,2, 朱金龙 1, 吴红艳 1, 徐坤 1, 翟红 1
|
A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed
|
Zhengjun Xia 1,*( ), Yuzhuo Li 1,2, Jinlong Zhu 1, Hongyan Wu 1, Kun Xu 1, Hong Zhai 1
|
|
图1. 种子钻孔和样品采集过程 (A) 种子组织的钻取; (B) 移液枪头收集种子组织; (C) 钻取种子组织并转移至384深孔板中; (D) 一列取样完成后贴胶带保护; (E) 钻头的清洁; (F) 移液枪头和手套的清洁; (G) 钻孔后的大豆种子; (H) 钻孔大豆种子播种后14天的幼苗。中间部分为整体流程简图, 种子取样经钻取、转移和清洁3个步骤, 再进行下一个种子的钻取。
|
Figure 1. Procedure for seed drilling and sample collection (A) Drilling of seed tissue; (B) Dilled tissue was collected by a pipette tip; (C) Transferring into a 384 deep-well plate; (D) When all wells in a row of the 384 deep-well plate were filled, the row will be sealed with a sticky tape; (E) Cleaning of the driller; (F) Cleaning of the tip and gloves; (G) Appearance of drilled seed; (H) Seedling of drilled seed (14 days after being sown). In the middle panel, the whole procedure is shown, in which seed sampling is performed by drilling, transferring and cleaning before proceeding to the next one.
|
|
|
|
|