图1. 靶向随机突变的双元载体图
(A) pH-STEME-1-esgRNA; (B) pH-STEME-NG-esgRNA。OsU3p: 水稻OsU3启动子; Ubip: 玉米Ubi-1启动子; A3A: 胞嘧啶脱氨酶APOBEC3A; ecTadA-ecTadA7.10: 腺嘌呤脱氨酶ecTadA-ecTadA7.10; NLS: 核定位信号; UGI: 尿嘧啶DNA糖基化酶抑制剂; E9t: 豌豆rbcS-E9终止子; 35Sp: 花椰菜花叶病毒35S启动子; HygR: 潮霉素抗性基因; CaMVt: 花椰菜花叶病毒终止子信号; LB: T-DNA左臂重复序列; RB: T-DNA右臂重复序列; L: 连接序列

Figure 1. Architectures of the binary vectors
(A) pH-STEME-1-esgRNA; (B) pH-STEME-NG-esgRNA. OsU3p: Rice OsU3 promoter; Ubip: Maize Ubi-1 promoter; A3A: Cytidine deaminase APOBEC3A; ecTadA-ecTadA7.10: Adenosine deaminase ecTadA-ecTadA7.10; NLS: Nuclear localization signal; UGI: Uracil DNA glycosylase inhibitor; E9t: Pea rbcS-E9 terminator; 35Sp: Cauliflower mosaic virus 35S promoter; HygR: Hygromycin resistance gene; CaMVt: Cauliflower mosaic virus terminator; LB: T-DNA left border repeat; RB: T-DNA right border repeat; L: Linker sequence